Figure 2
From: Revealing hidden complexities of genomic rearrangements generated with Cas9
Characterization of founder mouse #1 and its offspring mouse #2. (A) Fibre-FISH show the presence of wild type (WT) and inversion (INV) alleles as well as an allele showing a deletion and the inversion of the deleted Nox4 fragment as well as a duplication [Ex-DUP] of a region external to the gRNA cut site (DEL + Ex-DUP/INV). This allele was transmitted to offspring, mouse #2. (B) Representation of the DEL + Ex-DUP/INV allele. PCR primers F1/3 (green arrowhead) and F2/4 (blue arrowhead) are too far apart to generate a FW PCR product. The RV PCR has a 219 bp insertion between the 5′ and 3′ breakpoints consisting of fragments of sequences originating from either the bridge oligonucleotide (blue) or Nox4 (dark grey). The junction between the external duplication and the inverted Nox4 region is shown as a dotted line.
