Figure 4
From: Revealing hidden complexities of genomic rearrangements generated with Cas9
Analysis of founder mouse #4. (A) Fibre-FISH showing presence of a wild type (WT), deletion (DEL) and duplication (DUP2) allele. (B) Meta and interphase-FISH with two colours; probes 1 + 4 (external) are green while probes 2 and 3 (internal) are red, confirming the DEL allele (red arrowheads). (C) Illustration of the structure of the duplication allele (DUP2) observed in this mouse. Sequence of the RV PCR band identified a 26 bp insertion from the bridge oligo (blue), 128 bp of Nox4 (grey) and a 6 bp deletion. The junction between the inverted duplicates is shown as a dotted line.
