Table 1 Overview of mice analyzed using endpoint PCR.
From: Revealing hidden complexities of genomic rearrangements generated with Cas9
Junction | # 1 | # 2 F1 of # 1 | # 3 | # 4 | # 5 | # 6 | # 7 | # 8 | # 9 F1 of # 8 |
|---|---|---|---|---|---|---|---|---|---|
PCR 5′ | faint | faint | + | + | + | + | + | double | + |
PCR 3′ | + | + | + | + | + | + | + | + | + |
DEL | + | + | + | + | |||||
FW | + | ||||||||
RV | + | + | + | ||||||
DUP | + | + | |||||||
PCR genotype | WT, DEL, INV RV | WT, DEL, INV RV | WT, DEL, INV FW | WT, DEL, INV RV | WT | WT | WT | WT, DUP1 | WT, DUP1 |
