Table 1 Oligonucleotides used in site-directed mutagenesis.

Y101D-Forward

5′- GGATTATCCATGGGGAATGACATAGGCCTTATCAATAG -3′

Y101D-Reverse

5′- CTATTGATAAGGCCTATGTCATTCCCCATGGATAATCC -3′

Y101A-Forward

5′- GGATTATCCATGGGGAATGCCATAGGCCTTATCAATAG -3′

Y101A-Reverse

5′- CTATTGATAAGGCCTATGGCATTCCCCATGGATAATCC -3′

Y162D-Forward

5′- GGCCGCTAAACTTGCAGATCTTCAGATATTATCAG -3′

Y162D-Reverse

5′- CTGATAATATCTGAAGATCTGCAAGTTTAGCGGCC -3′

Y162A-Forward

5′- GGCCGCTAAACTTGCAGCTCTTCAGATATTATCAG -3′

Y162A-Reverse

5′- CTGATAATATCTGAAGAGCTGCAAGTTTAGCGGCC -3′

SIM102-Forward

5′-GGGAATTACATAGGCCTTGCCAATAGAATTGCCCAG-3′

SIM-102-Reverse

5′-CTGGGCAATTCTATTGGCAAGGCCTATGTAATTCCC-3′

SIM-163-Forward

5′-GCATATCTTCAGATAGCATCAGAAGAAACCTCAG-3′

SIM-163-Reverse

5'-CTGAGGTTTCTTCTGATGCTATCTGAAGATATGC-3′