Figure 8

Fenugreek supplementation did not increase beta cell mass or circulating insulin and C-peptide levels. Histology sections were performed on the pancreas of mice exposed to HF or HFFG diets for 16 weeks. βeta cells were stained for insulin and counter stained with hematoxylin and eosin (H&E). In mice injected with saline or insulin⁶³ for 10 minutes, serum was collected for insulin and C-peptide analyses via ELISA assay (a) Representative micrographs (10x) of insulin positive (brown) beta-islet cells. (b) Quantification of insulin-positive beta cell-islet to tissue fraction (c) Insulin, and (d) C-peptide ± insulin injection (1 U/kg). Data are represented as means ± SEM, (n = 6, 8 , &10), as indicated. Comparisons were made by a 1-way ANOVA followed by student t-test. *p < 0.05 **p < 0.01, ***P < 0.001 was considered significant. Scale bar = 10 microns.