Figure 9
From: Fenugreek supplementation during high-fat feeding improves specific markers of metabolic health
Fenugreek supplementation did not affect hepatic lipid accumulation, but substantially decreased liver FABP4/aP2/expression in HF-fed mice. The livers of mice exposed to HF or HFFG diets for 16 weeks were fixed and H&E stained, or collected for Western blot and tissue triglyceride analysis. (a) Representative photomicrographs of livers stained with H&E. (b) Quantification of liver tissue triglyceride content. (c) Western Blots of liver tissue extracts. The expression of fatty acid synthase (FAS)64, /fatty acid-binding protein 4 (FABP4/aP2), and extracellular signal–regulated kinases (ERK 1 and 2)62 is shown. Each lane represents a liver tissue extract from an individual animal. (d) FABP4 quantification normalized to ERK 1. Data are presented as mean ± SEM, (n = 6). Comparisons were made by a 1-way ANOVA and/or by student t-test. *p < 0.05 **p < 0.01, ***P < 0.001 was considered significant. Scale bar = 10 microns.
