Figure 6

Activation of complement (classical) and coagulation cascades. (A) A bar graph representing the comparison of the relative abundance of proteins involved in the complement and coagulation cascade. The x-axis represent the average fold changes (p-value ≤ 0.05 and ≥1.3-fold change, n = 10). (B) A schematic diagram showing the details of the three pathways activating the complement cascade (classical, lectin and alternative), of which our quantitative proteomics data supports the activation of the classical pathway in both retina and vitreous. ★, indicates differentially regulated proteins that are part of the classical pathway (C) Western blotting analysis measuring the protein level of CLU (retina and vitreous, n = 10) and VTN in vitreous (n = 10) of glaucoma and control samples. GAPDH was used as the loading control. The bar graphs indicate average densitometry measurements (ImageJ software) (n = 10, average ± sd, *p-value < 0.05). Black bars represent control, while the light grey bars represent glaucoma. The copyright of the basic complement pathway image belongs to Noris, Mescia, & Remuzzi, Nat. Rev Neprh. 2012.