Figure 2 | Scientific Reports

Figure 2

From: Effect of Specific Mutations in Cd300 Complexes Formation; Potential Implication of Cd300f in Multiple Sclerosis

Figure 2

Analysis of the effect of disruption of clusters 3 and 5 in CD300b complexes formation. COS-7 cells were transiently co-transfected with a combination of HA- and Flag-tagged CD300 constructs. Cell were lysed and immunoprecipitated with anti-HA.11 mAb. Blots were probed with the indicated antibodies in order to determine the capability of receptors to form complexes. Whole cell lysates (2%) were included as loading and transfection controls. (A) HA-CD300b wt was tested against Flag-CD300b R95G-Q97G and Flag CD300b E28A-Q29G. (B) HA- and Flag-tagged CD300b wt were tested against HA- and Flag-tagged CD300b E28A-Q29G. HA-CD300e and HA CD300d (C) and HA-CD300a and HA-CD300f (D) were tested against Flag-CD300b E28A-Q29G. Descriptive data were expressed as the arithmetic mean ± SD. The GraphPad Prism statistical package was used to investigate group differences by unpaired, two-tail Student’s t test. p values are indicated for statistically different means: * ≤ 0.05, ** ≤ 0.01, **** ≤ 0.0001. n.s: non-significant.

Back to article page