Figure 5
SNP identification in CD300f receptor. (A) Sequence chromatograms showing the genomic DNA (left) and vector-cloned cDNA (right) CD300f sequence covering SNP rs141171369 (17:72700901 C/T) identified in LF6105 patient. CD300f codon 33 has been underlined. (B) Protein sequence alignment of human CD300b and CD300f Ig-like domains. Identical residues are shown on black background. Cysteine residues involved in the Ig-like domain fold are identified with an asterisk. Secondary structure elements are shown above amino acid sequences. Residues Gln29 in CD300b and Arg33 in CD300f are highlighted with a green arrow. (C) COS-7 cells were transiently transfected with HA-CD300f wt in combination with Flag-CD300f wt or Flag-CD300fR33Q. Cells were lysed and immunoprecipitated with anti-HA.11 mAb. Blots were probed with the indicated antibodies.
