Figure 6
Functional consequences of SNP rs141171369 in CD300f. (A) Monocytes from a healthy individual (LF5995, right) and the MS patient (LF6105) were stained with anti-CD300f monoclonal antibody (UPD2) (light gray histogram) and an isotypic mAb as a negative control (dark gray histogram). (B) COS-7 cells were transiently transfected with Flag-CD300f wt or Flag-CD300fR33Q and CD300f cell surface expression was monitored 48 hours post-transfection as described above (% of CD300f positive cells and mean fluorescence intensity are shown) or (C) protein synthesis was inhibited by adding cycloheximide to the cell culture for the indicated time-points. Cells were lysed and cell lysates were subjected to western blot as described. Receptor half live was calculated using GraphPad Prism (phase decay non-linear regression) and differences were investigated by unpaired, two-tail Student’s t test. p values are indicated for statistically different means: *** ≤ 0.001.
