Figure 2
From: Impact of Endothelial Lipase on Cholesterol Efflux Capacity of Serum and High-density Lipoprotein
In vitro EL-modification of serum decreases HDL size and generates lipid-free/-poor apoA-I (a) Concentrations of HDL-particles (HDL-p) in hEV-serum and hEL-serum determined by NMR spectroscopy. Western blotting (apoA-I) following non-denaturing 4–16% polyacrylamide gel electrophoresis of: (b) hEV-serum and hEL-serum and (c) hEV-apoB-DS and hEL-apoB-DS. Protein size annotations refer to protein marker bands on the membranes. The arrows indicate the position of lipid-free/-poor apoA-I. Results in (a) are mean ± SEM of 2 modifications of pool-serum from 8 donors, each measured in duplicates and analysed by unpaired t-test. Results in (b) and (c) are representative of 5 different modifications of the pool-serum. *P < 0.05, ***P < 0.001.
