Figure 2
The heat shock response is impaired in the R6/2 mouse model of Huntington’s disease. (A) Heat map showing the basal transcript levels of several heat shock response genes and regulators in cortex, liver, quadriceps femoris (quad. fem.) and tibialis anterior (tib. ant.) muscles at different stages (E14.5 = embryonic day 14.5; p7 = postnatal day 7; wk = weeks). Data are the log2 fold changes of R6/2 vs. wild type mice. Data are mean ± SEM; n = 4; two-tailed homoscedastic Student’s t-test. Genotype: *p < 0.05, **p < 0.01, ***p < 0.001. (B) Transcript induction at 4 hours after heat shock or HSP90 inhibition (HSP990) in quadriceps femoris muscle of R6/2 and wild type mice at 12 week of age. Data are mean ± SEM relative to the levels of control or vehicle treated wild type animals; n ≥ 6; two-way ANOVA with Tukey post hoc test. (C and D) Heat shock protein induction at 24 hours after heat shock (HS) (C) or HSP90 inhibition (HSP990) (D) in quadriceps femoris muscle of R6/2 and wild type mice at 12 week of age. Data are mean ± SEM relative to the levels of control or vehicle treated wild type animals; n ≥ 6; two-way ANOVA with Tukey post hoc test. TUBA1a/b was used as a loading control. (B,C and D) Treatment: *p < 0.05, **p < 0.01, ***p < 0.001 and treatment/genotype # p < 0.05, ## p < 0.01, ### p < 0.001.
