Figure 3
Effect of metformin on BMP6-mediated SHP gene expression in WT and AMPKLiv KO mice. (A) Q-PCR analysis showing SHP mRNA levels in mouse primary hepatocytes treated with BMP6 (10 nM) or/and metformin (from 0.25 to 1 mM) for 24 h. RNA extraction and real-time quantification of transcripts were performed as described. mRNA expression was calculated using the ΔΔCt method and normalized to the expression of cyclophilin A. SHP gene expression in BMP6 treated hepatocytes, with or without metformin, is expressed as-fold SHP expression of that in non-treated hepatocytes. Error bars represent SD for n = 3 samples in each group. Statistical significance is indicated by * (*p < 0.05, **p < 0.005, ***p < 0.0005 as compared to the BMP6 condition).
