Table 3 Number of genes expressed in at least 10% of the cells in the simulated data sets, comprised of subsamples of the scRNA-seq data set 1, with various sequencing depths (columns) and read lengths (rows).
From: Impact of sequencing depth and read length on single cell RNA sequencing data of T cells
Sequencing depth (PE reads x million) | ||||
|---|---|---|---|---|
Read length (bp) | 0.05 | 0.25 | 0.625 | 1.25 |
25 | 6,081 | 8,497 | 9,184 | 10,849 |
50 | 5,665 | 7,801 | 8,255 | 8,240 |
100 | 5,141 | 6,879 | 7,333 | 7,440 |
150 | 4,836 | 6,458 | 6,824 | 6,936 |
