Figure 1
mRNA expression of APOBEC3 family members in liver biopsies of patients treated with IFN. (A) Meta analysis of transcription levels of APOBEC3 family members, in two studies reporting hepatitis C patients under IFN treatment13,14. The top scaled color bar indicates the relative gene expression level, and the dark and light blue bars illustrate two groups of patients: under and before therapy. In the heatmap, each column contains the data from one patient, and each square shows the gene expression level in a specific individual. All changes had FDR(BH) <0.01 and p after Bonferroni correction <0.05. More details for all six genes are summarized in Supplementary Table 3. (B,C) Total RNA from liver biopsies of naïve- or IFN-α-treated CHB patients were extracted. After reverse transcription, mRNA expression levels of APOBEC3A, -B, -C, -D/E, -G (B), -F, and -H (C) were quantified and normalized to GAPDH mRNA. Student’s t test was used to compare the two groups. *P < 0.05; N.S. no significance.
