Figure 5 | Scientific Reports

Figure 5

From: Non-muscular myosin light chain kinase triggers intermittent hypoxia-induced interleukin-6 release, endothelial dysfunction and permeability

Figure 5

IH increases early stages of atherosclerosis in a nmMLCK-dependent manner. (A) Analysis of adhesion of THP-1, a monocytic cell line, on HAoECs exposed to Nx or IH in absence or presence of ML-7 (5 µM). Images are representative of 3 independent experiments. Arrows show THP-1 cells. (B) Quantification of the adhesion assay. THP-1 cells per field were count on 4 different images per condition (n = 3). (C) Analysis of transmigration of THP-1 across a layer formed by HAoECs exposed to Nx or IH in absence or presence of ML-7 (5 µM) using Transwell. Histograms represent the number of THP-1 count in the lower chamber (n = 10). *P < 0.05, **P < 0.01, ***P < 0.001.

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