Figure 3 | Scientific Reports

Figure 3

From: CCAAT/Enhancer binding protein β silencing mitigates glial activation and neurodegeneration in a rat model of Parkinson’s disease

Figure 3

Injection of 6-OHDA in the Substantia nigra pars compacta (SNpc) of adult rats significantly induces C/EBPβ overexpression in a in vivo model of Parkinson’s disease. Rats were injected into the SNpc with 6-OHDA (5 μg). After 3, 7, and 30 days animals were sacrificed, and the brains processed for immunofluorescence, as indicated in Methods. (a) Triple immunofluorescence representative images of both hemispheres (contra- and ipsilateral) showing C/EBPβ expression (green) together with tomato lectin (red) an activated microglia marker and tyrosine hydroxylase (TH, blue). (b) Immunofluorescence images of both hemispheres (contra- and ipsilateral) showing C/EBPβ expression (green) together with GFAP (red) to label astroglial cells. Insets show high magnification of the selected area. Nuclei were stained with DAPI. Scale bar = 100 μm. (c) Quantification of the total number of dopaminergic neurons in the SNpc is shown. ***p ≤ 0.001, *p ≤ 0.05 versus control (PBS-injected animals).

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