Figure 5 | Scientific Reports

Figure 5

From: CCAAT/Enhancer binding protein β silencing mitigates glial activation and neurodegeneration in a rat model of Parkinson’s disease

Figure 5

Injection of lentiviral particles containing C/EBPβ shRNA in the Substantia nigra pars compacta of adult rats significantly reduces astroglial activation in a in vivo model of Parkinson’s disease. Rats were injected into the SNpc with lentiviral particles containing Non-Targeting shRNA (NT-shRNA) or C/EBPβ-shRNA in combination or not with 6-OHDA (5 μg). After 7 (a) and 15 (b) days animals were sacrificed, and the brains processed for immunofluorescence, as indicated in Methods. Double immunofluorescence representative images of both hemispheres (contra- and ipsilateral) showing C/EBPβ expression (green) together with the astroglial marker glial fibrillary acidic protein (GFAP, red). Nuclei were stained with DAPI. Insets show high magnification of the selected area. Scale bar = 100 μm.

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