Figure 5
From: Controlled delivery and minimally invasive imaging of stem cells in the lung
MSC delivery into the rat trachea and in situ visualization. (a) Photograph of the imaging setup for visualizing the rat trachea and (b) light-path diagram of the imaging setup. IP: imaging probe, OL: objective lens, DM: dichroic mirror, F1 & F2: optical filters 1 & 2, TL: tube lens. (c) Imaging probe characterization to visualize (d) (i) 1-μm, (ii) 10-μm particles, and (iii) MSCs suspended in liquid droplets and placed onto glass slides. Imaging probes (e) without and (f) with a microprism mirror attached that allow front- and side-view in the trachea, respectively. (g) (i) Bright-field and (ii) fluorescent images obtained using front-view imaging probe following delivery of Qdot-labeled MSCs into the rat trachea. TR: trachea. (h) (i) Fluorescent images of 10-μm particles, (ii) MSCs, and (iii) their seeding density Γ in the rat trachea.
