Figure 2

Impact of benchtop centrifugation on PoC finger-prick viral load quantification. Comparison of Roche TaqMan pVL assay with GeneXpert Viral Load assay without (a) or with (b) a 3 minute centrifugation step. Vertical line in (a) shows suggested lower limit of detection for the finger-prick protocol (1000 copies/ml) based on this study. Correlation between Roche TaqMan pVL assay and Xpert Viral Load assays when a lower limit of detection of 1000 copies/ml was imposed and 6 samples with pVL below this excluded (d). Values are Spearman’s Rho and P values. Bland Altman analysis comparing the Roche TaqMan with Xpert VL assay with centrifugation for samples > 1000 copies/ml (d).