Figure 5
ONAS does not change multipotency of NSCs during differentiation. (A–I) NSCs were expanded for 1 week as neurospheres, dissociated onto the flat surface or ONAS, and differentiated for 4 days in the absence of EGF and FGF2. Then, cells were fixed and immunostained with TuJ1 antibody (A–C, green), anti-GFAP antibody (D–F, red) or anti-GalC antibody (G–I, green). Nuclei were stained with DAPI (blue). Scale bar, 50 μm. Quantification of neurons (C), astrocytes (F) and oligodendrocytes (I). TuJ1-positive, GFAP-positive or GalC-positive cells were counted and normalized to total cell number to obtain percentage. The values were presented as mean ± s.e.m. (n = 4 for neurons and astrocytes, n = 3 for oligodendrocytes). Statistical analysis of data was performed using the Student’s t-test.
