Figure 7
ONAS does not change multipotency of NSCs (3.3 cells μl−1) during differentiation. (A) Experimental scheme. NSCs (3.3 cells μl−1) were cultured in the presence of mitogen for 5 days and differentiated for 3 additional days in the absence of EGF and FGF2. (B) At the 5th day of proliferation at 3.3 cells μl−1, NSCs on the flat surface grew individually, whereas NSCs on ONAS formed neurospheres. After 3 additional days of differentiation, NSCs on ONAS migrated out from the neurosphere and differentiated. Scale bar, 50 μm. (C) NSCs (3.3 cells μl−1) that proliferated differentially on the flat surface or ONAS were differentiated for 3 additional days, fixed and immunostained with TuJ1 antibody (a and b, green) and anti-GFAP antibody (d and e, red). Scale bar, 50 μm. Quantification of neurons (c) and astrocytes (f). Data were expressed as mean ± s.e.m. (n = 3). Statistical analysis was performed using the Student’s t-test.
