Figure 3

Peripheral structures of the Pithovirus particle imaged using energy-filtered cryo-EM at 200 kV. (A) The entire structure of the Pithovirus particle. Arrows indicate the low-density outer layer identified in this study. (B) Close-up view of the viral surface. The surface of the Pithovirus particle consists of four layers, those are, (a) nucleoid, (b) interior gap, (c) tegument and (d) low-density outer layer. (C) Close-up view of the apical cork. Arrows indicate vertical stripes that are formed at intersections of the horizontal parallel lines. Dark dots show fiducial markers of gold colloids.