Figure 5 | Scientific Reports

Figure 5

From: Structural variability and complexity of the giant Pithovirus sibericum particle revealed by high-voltage electron cryo-tomography and energy-filtered electron cryo-microscopy

Figure 5

Tomographic 3D reconstructions of the Pithovirus particles using cryo-HVEM at 1 MV. (A) Non-tilted image of ice-embedded Pithovirus particles. Arrows indicate the positions of the apical pores. (B) A Z-slice of the tomographic 3D reconstruction. (C–F) Structural segmentations of the entire 3D structures of the individual Pithovirus particles. Pithovirus particles with small length (C–D, particle 1), and medium length (E–F, particle 2). (C and E) Outer surface image of the segmentations. (D and F) Cross-section images of the segmentations. In the segmentation images, yellow, green, red, blue and pink surfaces indicate nucleoid, tegument, a root of the apical cork, apical cork, and low-density outer layer, respectively.

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