Figure 1 | Scientific Reports

Figure 1

From: Identification and characterization of a putative protein disulfide isomerase (HsPDI) as an alleged effector of Heterodera schachtii

Figure 1

Structure and functional annotation of HsPDI and expression pattern of HsPdi gene. (A) Detailed amino acid sequence of HsPDI protein with predicted signal peptide (grey), thioredoxin domains (a and a’ in red, b and b’ in green) and functional catalytic active site (asterisks). (B) In situ hybridization of DIG-labelled antisense HsPdi probe to pre-parasitic J2s showed transcripts localized inside the sub-ventral oesophageal gland (purple stain). The sense probe was used as control. Bars: 20 μm. (C) The relative expression levels of HsPdi mRNA quantified using qRT-PCR. The fold change values of changes in HsPdi mRNA abundance in pre-parasitic J2, J3, J4, and female relative its abundancy in eggs. Data are averages of three biologically independent experiments, each consisting of three technical replicates. H. schachtii actin was used as an internal control to normalize gene expression level.

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