Figure 6

HsPDI expression is triggered by H₂O₂ and increases H₂O₂ tolerance. (A) Mortality rate of freshly-hatched J2s in H₂O_2, n = 9. (B) The relative HsPdi mRNA expression levels in freshly hatched J2s quantified using qRT-PCR after soaking for 30 min in 5 and 10 mM H₂O₂. The fold change values were calculated in H₂O₂ incubated nematodes relative to J2s soaked in sterile distilled water (0 mM H₂O₂), n = 9. (C) Effect of HsPdi silencing on H₂O₂ stress tolerance, freshly hatched J2s of H. schachtii were soaked in HsPdi dsRNA or GFP dsRNA as a control. dsRNA-treated nematodes were soaked in 5 mM H₂O₂ or in sterile water and alive nematodes were counted after 30 min, n = 12. (D) ROS bursts in response to the bacterial elicitor peptide flg22 was measured in relative light units (RLU) in plants expressing HsPDI and compared with Col-0 using luminol-based assay after 120 min-long incubation, n = 12. Data are based on three independent experiments. Each bar represents the mean ± standard error. Asterisk marks indicates significant differences based on Student’s t-test (P < 0.05).