Figure 1
From: Protein interaction network of alternatively spliced NudCD1 isoforms
Alternative splicing results in three different NudCD1 isoforms. (A) Schematic representation of the first 4 exons of NudCD1 and the resulting isoforms that differs in their N-termimus, while all isoforms include exons 5 to 12. Isoform 1 consists of exons 1 and 3, isoform 2 consists of exons 2 and 3, while the isoform 3 includes the exons 1, 3 and 4, but uses an initiation codon in the fourth exon, resulting in a smaller protein. (B) Total protein lysates from U2OS FT cells with doxycycline-induced GFP-tagged NudCD1 isoforms were analyzed by Western blotting using a GFP antibody to confirm expression of the different isoforms following induction using doxycycline (lane 2, 4, 6). (C) Total protein lysates from U2OS FT cells with doxycycline-induced GFP-tagged NudCD1 isoforms treated or not with MG132 were analyzed by Western blotting using a GFP antibody.
