Figure 2
From: Protein interaction network of alternatively spliced NudCD1 isoforms
The different NudCD1 protein isoforms have distinct subcellular distribution. Fluorescence microscopy analysis of the U2OS FT cells expressing the respective GFP-fusion proteins was performed using an antibody to GFP (panels A, D and G), and the nuclei were stained with DAPI (panels B, E and H). NudCD1 Isoform 1 shows a strictly nuclear accumulation (A), NudCD1 isoform 2 shows both a cytoplasmic and nuclear (D) and a predominantly cytoplasmic accumulation is shown for NudCD1 isoform 3 (G). Cells transfected with a NudCD1 Isoform 1 with a mutated NLS (RVKR to AVAA) were labelled with an antibody to GFP (J), and the nuclei were stained with DAPI (K).
