Figure 3
From: Protein interaction network of alternatively spliced NudCD1 isoforms
Identification of NudCD1 isoform-specific interacting partners. NudCD1 interacting partners were identified using a combination of immunoprecipitation following by quantitative mass spectrometry analysis using triple-labeling SILAC. Cells not expressing NudCD1 were grown in “light” (L) media, cells expressing NudCD1 isoform 1 were grown in “medium” (M) media and cells expressing either NudCD1 isoform 2 or isoform 3 were grown in cells grown in “heavy” (H) media. Immunoprecipitation were performed using the recombinant GFP-Trap_A reagent by combining a control, isoform 1 and isoform 2 (A), or a control, isoform 1 and isoform 3 (B). The overlap between the proteins identified as interactors for each isoforms is displayed as a Venn diagram (C).
