Figure 7
From: Protein interaction network of alternatively spliced NudCD1 isoforms
DHX15 colocalizes with SF3 proteins in nuclear speckles, but not NudCD1. U2OS cells transfected with Myc-DHX15 and NudCD1-GFP constructions were fixed and revealed with GFP and Myc antibodies (Fig. 7A–D). To confirm that the localization pattern of DHX15 was consistent with splicing speckles, we also performed immunofluorescence experiments on DHX15 and one of the identified splicing factor, SF3A3 (Fig. 7E–H). The nucleus were stained with DAPI.
