Figure 1
Improvement of clinical score after RANTES-loaded microparticle injection. (a) Representative confocal microscopy image of pullulan/dextran-based microparticles (MP) incubated with avidin-FITC alone (Avidin-loaded control MP) or with 10 nM biotinylated-RANTES followed by avidin-FITC to assess RANTES localization. Scale Bar: 50 µm. (b) 10 nM biotinylated RANTES-loaded MP (15 µg/mL) were injected intramuscularly in 6-week old male BALB/c white mice into their ischemic limb. At day 0, RANTES-loaded MP were identified in the injection area (I), as indicated with arrows on hematoxylin-eosin stained sections of the ischemic tissue, observed with optical microscopy. Bar: 1 mm. (c) Histograms represent clinical scores calculated as the sum of the grade obtained for Tarlov score, ischemic score and modified ischemic score at days 0, 5 and 10 after hindlimb ischemia. The effect of two different ways of treatment administration by injection of PBS, VEGF (2 nM) or RANTES (10 nM) solution or by PBS-, VEGF- or RANTES-loaded MP intramuscular injection in ischemic limb was evaluated,*P < 0.05 versus PBS solution at day 10.
