Figure 5 | Scientific Reports

Figure 5

From: Structure and function of the alternatively spliced isoforms of the ecdysone receptor gene in the Chinese mitten crab, Eriocheir sinensis

Figure 5

Expression analysis of the four EcR isoforms in different tissues. (A) qRT-PCR analysis of the relative expression levels of Es-EcR-2, Es-EcR-3, and Es-EcR-4 in different tissues, normalized to the geometric mean of 3 control genes (β-actin, S27 and VATB). Error bars represent standard error. Groups marked by different lowercase letters represented significant difference at p < 0.05. (B) Semiquantitative PCR results for the expression levels of Es-EcR-1, Es-EcR-2, and Es-EcR-3 in the different tissues. (C) qRT-PCR analysis of the relative expression levels of Es-EcR-2 during different ovary developmental stages. Groups marked by different lowercase letters represented significant difference at p < 0.05. Hp: hepatopancreas, E: eyestalks, S: stomach, M: muscle, G: gill, H: heart, TG: thoracic ganglia O: ovary, and T: testis.

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