Figure 4

Immunohistochemical detection of Ov-TSP-2 and Ov-TSP-3 in Opisthorchis viverrini from the bile ducts of infected hamsters. Panels A and B: Liver sections of infected hamsters probed with pre-immunization sera showing no immunoreactivity. Panels C and D: Liver sections of infected hamsters probed with rabbit anti Ov-TSP-2 (C) and Ov-TSP-3 (D) sera showed positive staining in the tegument (T) and eggs (E) within the uteri of the flukes and in the biliary epithelium (B) of hamsters adjacent to the flukes but not in the gastrodermis (G) of the parasite.