Figure 5 | Scientific Reports

Figure 5

From: Human rickettsial pathogen modulates arthropod organic anion transporting polypeptide and tryptophan pathway for its survival in ticks

Figure 5

RNAi-mediated silencing of kat in tick cells affects A. phagocytophilum growth and isoatp4056 expression. QRT-PCR analysis showing expression of kat (A and C) or isoatp4056 (B and D) in A. phagocytophilum-infected mock or kat- (A and B) or kat + isoatp4056-dsRNA-treated (C and D) tick cells is shown. A. phagocytophilum burden in mock or kat- (E) or kat + isoatp4056-dsRNA-treated (F) tick cells is shown. Each circle represents data from one independent culture plate well. Black circles indicate data from mock-treated and gray circles represents data from either kat-dsRNA-treated or kat + isoatp4056-dsRNA-treated tick cells. The mRNA levels of kat or isoatp4056 are normalized to tick beta-actin levels and the levels of P44 (A. phagocytophilum burden) were normalized to tick 16S levels. P value from non-paired Student’s t-test is shown.

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