Figure 2

Representative microscopic images of rat brown adipocytes treated with the fluorescent polymeric thermometer. A differential interference contrast image (a), a fluorescence image (490 nm excitation, 525 nm emission), (b) and a fluorescence image (490 nm excitation, 605 nm emission) (c) of the cellular thermoprobe in rat brown adipocytes on day 8. A merged image of (b) and (c) with the sampling square of the measurement are also shown in (d). Scale bar: 40 µm.