Figure 4 | Scientific Reports

Figure 4

From: The thermogenic actions of natriuretic peptide in brown adipocytes: The direct measurement of the intracellular temperature using a fluorescent thermoprobe

Figure 4

The profile of the intracellular temperature change in rat brown adipocytes incubated with ANP. The intracellular temperature was indicated by the fluorescence ratio (605 nm/525 nm). The changes of the fluorescence ratio in rat brown adipocytes (day 8) after treatment with ANP (10−9 M, n = 5; 10−7 M, n = 6), isoproterenol (10−7 M, n = 6), or CL316,243 (0.5 µM, n = 4) were recorded every 6 minutes (Control, n = 6) at 35 °C (a) or 37 °C (b). The data represent the mean ± SEM. *P < 0.03 and **P < 0.01 versus the controls at each time point (one-way ANOVA followed by Bonferroni’s multiple comparisons test).

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