Figure 5 | Scientific Reports

Figure 5

From: The thermogenic actions of natriuretic peptide in brown adipocytes: The direct measurement of the intracellular temperature using a fluorescent thermoprobe

Figure 5

ANP increases the UCP1 levels in rat brown adipocytes. The quantification of the Ucp1 gene expression levels in rat brown adipocytes (day 7 or 8) after one hour of incubation at 35 °C with ANP (10−7 M), isoproterenol (10−7 M), or CL316,243 (0.5 µM) (n = 3 each) (a) and those after 30 minutes of incubation at 35 °C with ANP (10−7 M) (n = 3) (b) were shown. The Ucp1 gene expression levels after one hour of incubation at 37 °C with ANP (10−9 M or 10−7 M, n = 4), isoproterenol (10−7 M, n = 4), or CL316,243 (0.5 µM) (n = 3) (c) and those after 30 minutes of incubation at 37 °C with ANP (10−7 M) (n = 3) (d) were also shown. The qPCR data were normalized to GAPDH. The data are shown as the fold change normalized to the levels found in untreated cells (control) *P < 0.05, **P < 0.03, ***P < 0.01 versus control (unpaired two-tailed Student’s t-test). (e) The ATP content in the adipocytes was measured after one hour of incubation with either ANP or isoproterenol (n = 3). Iso, isoproterenol, CL, CL316,243.

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