Figure 6
Thermogenic actions of ANP in rat brown adipocytes through p38MAPK-UCP1 pathway. (a) Phosphorylation of p38MAPK was evaluated in rat brown adipocytes treated with or without ANP (10−7 M) for one hour. Representative immunoblots obtained using the indicated antibodies are shown and full-length blots are presented in Supplementary Fig. S6. Averaged densitometry data normalized to the control at the same time points are shown in the bar graphs (n = 3). *P < 0.05 versus control. (b) The quantification of the Ucp1 gene expression levels in rat brown adipocytes (day 7 or 8) after one hour of incubation at 35 °C with or without ANP (10−7 M) stimulated with either SB203580 or DMSO (n = 3) are shown. The qPCR data were normalized to GAPDH. The data are shown as the fold change normalized to the levels found in untreated cells (control). **P < 0.01 versus control; ††P < 0.01 versus ANP + SB. (c) The changes of the fluorescence ratio in rat brown adipocytes (day 8) after treatment with or without ANP (10−7 M) stimulated with either SB203580 or DMSO were recorded every 6 minutes at 35 °C (n = 3). The data represent the mean ± SEM. **P < 0.01 versus control; †P < 0.03 and ††P < 0.01 versus ANP + SB at each time point (one-way ANOVA followed by Bonferroni’s multiple comparisons test). Con, control, SB, SB203580.
