Figure 3
Schematic representation of the experimental design used to test the duration of poly(I:C) priming and the efficiency in the environment. The same batch of oysters was anaesthetized before being injected with poly(I:C) or filtered seawater (FSW). Four priming experiments were performed at different times: at 126 days (priming A), 99 days (priming B), 71 days (priming C), 24 h (priming D) before immersion in the Thau lagoon (Mediterranean Sea). For priming A, the antiviral effect of the treatment was tested at 1 day, 14 days, 28 days, 56 days or 126 days post-treatment in experimental infections with OsHV-1. Throughout all the experiments, a batch of oysters remained untreated as a control. During each experimental infection, mortalities were monitored daily post-challenge. During the environmental test, mortalities were monitored daily and sampling performed when mortalities occurred (9 and 14 days post-immersion).
