Figure 4
Duration of poly(I:C) priming. Kaplan–Meier survival curves were generated from spats primed by injection after anesthesia with poly(I:C) (19 µg.g−1 of oyster) or with filtered seawater as control and injected 1 day (a), 14 days (b), 28 days (c), 56 days (d) or 126 days (e) post-priming A with OsHV-1 µvar homogenate (7.7 × 106 and 4.4 × 106 copies of DP gene per oyster, respectively) or a pathogen free control homogenate. One control conditions without injection is represented as non-treated (black line). Conditions demonstrating 100% survival rates can appear hidden and merged with other ones reaching the same survival rate. Mortalities in each group of 45 oysters (15 per tank) were monitored for 10 days after infection. a, b indicate p-value < 0.0001; log-rank test (n = 45).
