Figure 6
Poly(I:C) persistence in seawater and oyster hemolymph. Immuno-northern blotting using antibodies against double stranded RNA. Poly(I:C) was incubated for 0, 24 h, 48 h with sterile water (W), seawater (SW) or oyster plasma (P) (cell free hemolymph) and 1 µg of each sample separated in 1% agarose non-denaturing gel. A SmartLadder (MW-1700-02, Eurogentec) was used. Sterile water, seawater and plasma without addition of poly(I:C) were used as negative controls. After electrophoresis, (a) nucleic acids were stained using GelRed (image partially cropped) or (b) blotted onto a nylon membrane and immuno-localized using the J2 antibody (Scicons) (image partially cropped). For immune-blotting, 1 µg of C. gigas genomic DNA (gDNA) was added to the gel in place of the ladder, as a negative control. Full-length blots/gels are presented in Supplementary Figure 2S.
