Figure 2
EtDO-P4 treatment increases rhEPO sialylation. (A) Total GSLs extracted from cells were subjected to TLC followed by staining with orcinol/sulfuric acid. Lane 1, GSL standards; Lane 2, GSLs extracted from EC2-1H9 cells; Lane 3, GSLs extracted from EC2-1H9 treated with EtDO-P4. (B) rhEPO was purified using immunoprecipitation with an anti-EPO antibody. Sialylation was determined using WGA lectin. (C) Quantification of data in B; n = 3. **P < 0.01 (Two-tailed Student’s t-test). Data are presented as means ± S.E.M.
