Figure 3
Transient repression of UGCG increases rhEPO sialylation. (A) Total GSLs extracted from cells were subjected to TLC followed by staining with orcinol/sulfuric acid. Lane 1, GM3 standard; Lane 2, GSLs extracted from EC2-1H9 cells; Lanes 3–5, GSLs extracted from EC2-1H9 cells treated with siRNAs #1–3; n = 3. (B) Quantification of total GSLs. n = 3. (C) rhEPO was purified using immunoprecipitation with an anti-EPO antibody. Sialylation was determined using MAL I lectin. (D) Quantification of data in (C); n = 3. *P < 0.05, **P < 0.01 (Two-tailed Student’s t-test). Data are presented as means ± S.E.M.
