Figure 5 | Scientific Reports

Figure 5

From: Enhancing the sialylation of recombinant EPO produced in CHO cells via the inhibition of glycosphingolipid biosynthesis

Figure 5

EC2-1H9-miUGCG cells produce rhEPO with increased levels of sialylation. (A) EPO production was determined for 4 days of culture. Closed circles, EC2-1H9 cells; Open circles, EC2-1H9-miUGCG cells. (B) Intracellular CMP-Neu5Ac levels were determined by HPLC. (C) rhEPO was purified using an immunoaffinity column with an anti-EPO antibody. Sialylation was determined using MAL I lectin. (D) Molar ratio of sialic acid on rhEPO. n = 3. **P < 0.01 (Two-tailed Student’s t-test); NS, not significant. Data are presented as means ± S.E.M.

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