Figure 6
Analysis of sialylated N-linked glycans on rhEPO. (A) N-glycans released from rhEPO were labeled with 2-AB. 2-AB-labeled N-glycans were separated using a DEAE column by HPLC. 2-AB-labeled N-glycans of fetuin (top), rhEPO produced by EC2-1H9 cells (middle), and rhEPO produced by EC2-1H9-miUGCG cells (bottom). (B) Sialylated rhEPO determined in A. (C) Neutral, mono-, di-, tri-, and tetra-sialylated N-glycans of rhEPO quantified in A. n = 3. **P < 0.01 (Two-tailed Student’s t-test). Data are presented as means ± S.E.M.
