Table 1 Statistics after trimming and quality filtering as well as of de novo assembly.
P. longirostris | P. macrodactylus | |
|---|---|---|
After trimming and quality filtering | ||
Number of clean reads | ||
- control group | 35,357,808 | 38,963,116 |
- treatment group | 36,588,780 | 41,092,214 |
Number of nucleotides | ||
- control group | 3,180,202,720 | 3,506,680,440 |
- treatment group | 3,292,990,200 | 3,698,299,260 |
Average G | 46.9% | 49.8% |
Average C | 42.4% | 49.2% |
De novo assembly | ||
Total number of unigenes | 72,573 | 33,274 |
Total length of unigenes (nt) | 40,780,267 | 19,319,528 |
Mean length of unigenes (nt) | 562 | 581 |
N50 | 728 | 768 |
Distinct clusters | 15,631 | 5,400 |
Distinct singletons | 56,942 | 27,874 |
