Table 1 Effect of ionic strength on kinetic and equilibrium parameters of the interaction between wheat ATI and TLR4.

From: Interfering with the high-affinity interaction between wheat amylase trypsin inhibitor CM3 and toll-like receptor 4: in silico and biosensor-based studies

Ionic strength conditions

kass (M−1s−1)

kdiss (s−1)

KD (M)

20 mM PBS (no NaCl)

43000 ± 2000

0.0001 ± 0.00005

(2.3 ± 1.2) × 10−9

20 mM PBS (25 mM NaCl)

39000 ± 4000

0.0004 ± 0.0002

(1.0 ± 0.5) × 10−8

20 mM PBS (50 mM NaCl)

40000 ± 2500

0.0007 ± 0.0001

(1.8 ± 0.3) × 10−8

20 mM PBS (140 mM NaCl)

41000 ± 6000

0.0025 ± 0.0006

(6.1 ± 1.7) × 10−8

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