Figure 5

Cryo-EM images of sonicated non-crosslinked nanobubbles. (A–C) Disrupted nanobubbles are shown colorized for better visualization. Typically, disrupted nanobubbles are observed along the somewhat hydrophobic carbon support layer of the EM grid (dark gray). Both deformed sheet-like structures and dot-like objects are observed suggesting the instability of nanobubbles after sonication. (D–F) Non-colorized versions of panels A-C. Scale bars, 100 nm.