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Figure 1

From: Synchrotron radiation circular dichroism spectroscopy reveals structural divergences in HDL-bound apoA-I variants

Figure 1

ApoA-I proteins analyzed in the lipid-free and lipid-bound states. Wildtype (WT), Milano (M) and A164S (A) apoA-I proteins were analyzed in the lipid-free (a) and lipid-bound (b,c) states. (a) SDS-PAGE analysis of proteins’ homogeneity before HDL formation. (b,c) Native gel analysis (left panel) and SDS-PAGE analysis (right panel) of proteins in the lipid-bound state before (a) and after (b) size exclusion chromatography. 2.5 µg of protein was loaded per lane in both SDS-PAGE and native gel analysis.

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