Table 1  Melting temperatures (Tm) calculated for the thermal unfolding of the ApoA-I proteins. Tm were calculated by fitting the experimental data with a biphasic non-linear regression. Data shown are the mean ± SD.

From: Synchrotron radiation circular dichroism spectroscopy reveals structural divergences in HDL-bound apoA-I variants

 

lipid-bound (current study)*

Lipid-free

Tm1

Tm2

Tm

ApoA-I WT

46.6 °C ± 3.6 °C

78.8 °C ± 0.2 °C

57.3 °C ± 0.4 °C31 55.9 °C ± 1.4 °C30 55.3 °C ± 0.5 °C42

ApoA-I Milano

55.9 °C ± 5.0 °C

72.15 °C ± 0.4 °C

50.9 °C ± 1.4 °C30

ApoA-I A164S

55.69 °C ± 6.1 °C

78.3 °C ± 0.2 °C

57.2 °C ± 0.2 °C31

  1. *R square for the biphasic fitting of the experimental data points: 0.9914, 0.9916 and 0.9911 for WT, Milano and A164S apoA-I proteins, respectively.
Back to article page